Oil & Grease Test for the Histosol Op-GrzBio Product Report O&G ? 1 ? , ? 4 ? Experiment Condition: Acid used: Enzyme used: Experiment Date: Experiment Method: Experiment Temperature: Sample used: Solvent used: Technique included: Condition enzymatic reaction: Condition for sample collection: Dosage Method: Procedure: 1:1 HCl HISTOSOL OP-GRZBIO (OP-Bio10) 14th to 29th Oct APHA Room Temperature LG 7 wastewater 8:2 ; n-Hexane : Methyl-tert-butyl ether Centrifugation, Distillation and Separation 

The oil tank was in running water and the enzyme was inputting into the oil tank continuously The sample was collected at two time slogs: 1) Between 13:00~13:30 2) Between 18:00~18:30 1st Day dosage method: -1L OP-Bio10 enzyme into 0.99m3 oil tank 2nd Day and onwards dosage method: -800ml OP-Bio10 into 4L water for 4 hours running Water running in the oil tank: -around 1m 3 / hr 500m l of wastewater was drawn out from the oil tank. The mixture was then mixed with the 1:1 HCl until the pH 2 condition is established. Separation with the separating funnel with the 30ml solvent used. 

Repeat for 3 times. Use the Na2SO4 to dry the organic layer. Transfer the organic layer into the weighted RB flask. Distillate the solvent out from the mixture. Weight the RB flask again and record the weight difference. Oil & Grease Test for the Histosol Op-GrzBio Product Report O&G ? 2 ? , ? 4 ? Data Record and Calculation: Date Time 12:00 19:00 14:20 18:30 15:22 16:28 13:32 14:35 19:45 14:00 18:00 12:13 14:13 14:00 18:20 27th Oct 18:20 = mg = mg = X 1000 = mg / L mg of oil and grease per liter From the Blank : mg of oil and grease / L 185.2 185.2 mg Blank for Evening 193.0 Blank for Noon 177.4 Around up for the Blank = This data is from the enzymes (OP-Bio10 & DX) Sample Tested Noon Sample 

Tested Evening Initial weight of the RB flask (g) Final weight of the RB flask (g) Net weight of the RB flask (mg) 19th Oct 12:00 Blank 01_N 113.1354 113.3104 175.0 Blank 01_N 122.8852 123.0644 179.2 20th Oct Blank 02_N 123.0644 123.2544 190.0 Blank 02_E 114.5661 114.7591 193.0 22nd

 Oct Blank 03_N 99.1230 OP-Bio10_N 93.6228 99.2640 141.0 Blank 03_E 104.2928 104.3606 67.8 93.7328 110.0 OP-Bio10_N 96.2260 96.3480 122.0 23rd Oct Blank 04_N 99.1303 OP-Bio10_E 96.2585 99.2956 165.3 OP-Bio10_N 104.2909 104.5464 255.5 96.3275 69.0 24th Oct OP-Bio10_N 112.8136 112.8740 60.4 OP-Bio10_E 117.6928 117.7902 97.4 25th Oct Blank 05_N 93.6160 93.7220 106.0 OP-Bio10_N 104.2980 104.3470 49.0 26th

 Oct OP-Bio10_N 114.5778 114.7918 214.0 OP-Bio10_E 115.9840 116.0637 79.7 OP-Bio10_E 115.9870 116.0168 29.8 370.37 500 Oil & Grease Test for the Histosol Op-GrzBio Product Report O&G ? 3 ? , ? 4 ? Average Reading for the OP-Bio10 g = - = % Average Reading for the OP-Bio10 g = - = % Average Reading for the OP-Bio10 g = - = % X 100% 185.2 71.47 X 100% 185.2 59.18 Third Day Dosage 116.0 X 100% 185.2 37.36 Calculation of the % of removal efficiency Enzyme used: OP-Bio10 First Day Dosage 116.0 % of removal efficiency 185.2 Second Day Dosage 75.6 % of removal efficiency 185.2 75.6 52.8 % of removal efficiency 185.2 52.8 Oil & Grease Test for the Histosol Op-GrzBio Product Report O&G ? 4 ? , ? 4 ? Conclusion and Forecast: From the data, it is observed that the enzyme OP-Bio10 showed a continuous trend in oil removing ability. The trend is shown as: Dosage Period Stage 1 Stage 2 Stage 3 59.18 71.47 Removal Efficiency 37.36 Removal Efficiency Vs OP-Bio10 Dosage Stages 0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00 Stage 1 Stage 2 Stage 3 Removal Efficiency (%) From the graph, it shows that there is an increasing trend of oil removal efficiency. It demonstrates that the addition of the OP-Bio10 is effective in oil removal. And the effectiveness is increase continuously and can reach to around 70% after the third day addition.